OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), 96T
Product details
The OxiSelect™ In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
Assay Principle.
Hydrogen Peroxide Standard Curve.
Detection of Various Free Radical Species. DCF fluorescence curves for AAPH (peroxyl radical generator), SIN-1 (peroxynitrite generator), and SNP (nitric oxide generator).