Description
HyperScribe™ Poly (A) Tailing Kit is a set of reagents designed to add a ≥150 base poly (A) tail to RNA transcripts generated with the HyperScribe™ T7 High Yield RNA Synthesis Kit. The kit is completed by using E. coli Poly (A) Polymerase (E-PAP) and ATP. Then the resulting capped and tailed RNA can be used in transfection or micro-injection experiments where mRNA stability and translation efficiency may be increased relative to unmodified mRNA.
Storage
Components and Storage
| Amount | Component | Storage |
| 100 uL | E-PAP (2 units/uL) | -20°C |
| 600 uL | 5X E-PAP Buffer | -20°C |
| 100 uL | ATP Solution (100 mM) | -20°C |
| 250 uL | 25 mM MnCl2 | -20°C |
| 1 mL x 2 | Nuclease - free Water | any temp* |
* Store Nuclease-free Water at -20°C, 4°C, or room temperature.