Epstein-Barr Virus (EBV) Lysate (1 mg)
Epstein-Barr Virus (EBV) is a human herpes virus (HHV-4) and has a diameter of approximately 120 to 180 nm. It is an enveloped icosahedral virus that contains a nucleocapsid and double-stranded linear DNA.
EBV (Strain: B95-8) is propagated in a transformed marmoset leukocyte cell line.
This virus is purified using sucrose density gradient ultracentrifugation, disrupted in the presence of 0.5% Triton X-100 non-ionic detergent/0.6 M KCl, and heat inactivated.
Viral lysate is usually sold in a vial containing 1.0 mg of protein, and is shipped on dry ice. Protein concentrations generally range from 0.5 to 3.0 mg/mL.
Custom orders are available, including specific buffer formulations and package sizes.
This product is intended for research, product development, quality assurance testing, or further manufacturing use.
Viral lysates can be utilized as an antigen, as a source for the purification of viral proteins, or for the detection of viral antibodies. Applications include:
- Immunodetection of antibodies to EBV using solid-phase enzyme immunoassays (EIA)
- Western blot
- Dot blot
- Other protein-based assays
ETIOLOGIC STATUS/BIOHAZARD TESTING:
EBV is a Biosafety Level 2 organism.
Viral inactivation of EBV lysate is validated by the absence of viral growth for other Human Herpes viral lysates tested in tissue culture based infectivity assays.
USE UNIVERSAL PRECAUTIONS when handling this product!
Viral lysate has been treated by a method validated to be effective for virus inactivation. However, no method can be guaranteed 100% effective.
This material should be handled as if capable of transmitting infectious agents.
Viral lysate should be stored at -65°C or below.
To avoid repeat freeze-thaws, which could negatively impact product performance, viral lysate should be stored in aliquots upon receipt.
DO NOT USE IN HUMANS. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
These products are NOT intended for use in the
manufacture or processing of injectable products
subject to licensure under section 351 of the Public
Health Service Act, or for any other product intended
for administration to humans.