Recombinant GFP, C-terminal 6xHis-tag - 100 μg/vial at 1.0 mg/mL in 1X PBS

Recombinant GFP, C-terminal 6xHis-tag - 100 μg/vial at 1.0 mg/mL in 1X PBS

Product details

Background

Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea victoria. Molecular cloning of the GFP gene and its subsequent expression in heterologous systems have established recombinant GFP (rGFP) as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. GFP can fuse with proteins of interest without interfering significantly with their assembly and function. Based on the structure of the GFP molecule, many GFP variants have been created with much improved fluorescence emission, or shifted excitation or emission spectra that are well suited for fluorescence microscopy and flow cytometry. Since rGFP requires no additional substrates or cofactors, rGFP fluorescence can be easily detected under fluorescence microscope after expression in either prokaryotic or eukaryotic cells.