AAV-2 Bicistronic Expression System (IRES-GFP) - 1 kit

• Complete Expression System contains packaging plasmids, expression vector and GFP control vector for packaging of recombinant AAV serotype 2
• pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
• Cloning capacity of expression vector = 1.7 kb

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells.