Anti-HLA-G (human) monoclonal antibody [Clone: MEM-G/9], FITC conjugated - 0.1 mg

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    Antigen: HLA-G


    Clone: MEM-G/9


    Conjugation: FITC


    Reactivity: Human


    Application: FC (QC tested)


    Application details: Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JEG-3 human choriocarcinoma cell line.


    Excitation wavelength: blue (488 nm)


    Isotype: Mouse IgG1


    Specificity: The antibody MEM-G/9 reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).


    Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.


    Concentration: 1 mg/ml


    Preparation: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.


    Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide


    Storage and handling: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.