Recombinant Human Legumain (LGMN) - 100 ug

Purity: Greater than 90% as determined by SDS-PAGE.

Target Names: LGMN

Uniprot No.: Q99538

Research Area: Immunology

Alternative Names: AEP; Asparaginyl endopeptidase; cysteine 1; Cysteine protease 1; EC 3.4.22.34; Legumain; LGMN; LGMN_HUMAN; LGMN1; Protease; Protease cysteine 1 (legumain); Protease cysteine 1; PRSC1

Species: Homo sapiens (Human)

Source: E.coli

Expression Region: 18-323aa

Target Protein Sequence:

VPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTN

*Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight: 38.8kDa

Protein Length: Full Length of Mature Protein

Tag Info: N-terminal 6xHis-tagged

Form: Liquid or Lyophilized powder

*Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.

Note: If you have any special requirement for the glycerol content, please remark when you place the order.

If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel: