Description: Purified, tag-free Staphylococcus aureus Gamma Hemolysin C (Hlg C) expressed in E. coli. The theoretical molecular weight of the protein is 32.8 kDa.
Storage: 2-3 weeks at -20◦C, -80◦C long term
Size: 100 µg of protein is supplied in PBS + 5% Glycerol at a concentration of 1.496 mg/mL. Protein demonstrates a molecular weight running close to 30 kDa.
Relevance: This protein may be used in functional toxicity assays in combination with Hlg B, or as a control protein in ELISA assays or Western blotting.
Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with Hlg B in a concentration range of 0.03-200 nM.