1 kb DNA Ladder 2.0 (0.5 kb ~ 10 kb, with loading dye)-100 rxn

1 kb DNA Ladder 2.0 (0.5 kb ~ 10 kb, with loading dye)-100 rxn

Cat. #: W108-100 (100 rxn); W108-500 (500 rxn)

Storage: Ship at 4℃. Store at -20℃ for up to 2 year. For frequent use, store at 4℃ for up to 6 months. Avoid freeze-thaw cycles.

Product Components

Product Description

1 kb DNA Ladder 2.0 is a mixture of 8 fragments: 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb. The 5 kb band is brighter than other bands as internal reference indicators. This product contains 1x Loading Buffer and can be directly load to gel. When loading 5 µL, the 5 kb band contains 150 ng DNA, and other bands contains 50 ng DNA.

Concentration: 500 ng / 5 μL

This product is for research use only.

Note:  

1. Do not heat before loading.

2. Please use fresh electrophoresis buffer and agarose gel for the best result.

3. High quality agarose should be used to avoid effecting the DNA bands and the recommended agarose gel concentration is 0.7% ~ 1%, recommended voltage is 4 ~ 8 v / cm.

4. This product is not for diagnostic use. The safety and efficacy of this product in diagnostics or other clinical uses have not been established.

Protocol  

Per reaction, load 5 µL of 1 kb DNA Ladder 2.0 on agarose gel to the well of agarose gel. If the well is wider than 6mm, increase the sample volume proportionally (1 µL / mm). Run electrophoresis.

The gel image shown is 5 µL of 1 kb DNA Ladder 2.0 separated by 0.7% agarose gel.