Assay range: 1.5 μg/L - 80 μg/L
Sensitivity: 0.5 μg/L
Standard:90 μg/L
Principle:
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to OxLDL-IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for OxLDL-IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain OxLDL-IgM and HRP conjugated OxLDL antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of OxLDL-IgM. You can calculate the concentration of OxLDL-IgM in the samples by comparing the OD of the samples to the standard curve.