Human Histone-binding protein RBBP7 ELISA kit - 96 wells plate
Detect Range: 78-5000 pg/mL
Target Protein Name: Histone-binding protein RBBP7
Sample type: serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type: Sandwich ELISA Kit
ELISA Test Principle:
The microtiter plate provided in Human Histone- binding protein RBBP7, RBBP7 ELISA KIT has been pre-coated with an Histone-binding protein RBBP7(RBBP7 antibody specific to Histone-binding protein RBBP7(RBBP7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histone-binding protein RBBP7(RBBP7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone-binding protein RBBP7(RBBP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histone-binding protein RBBP7(RBBP7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.